The Gran Fondo Novi Sad commences in front of the Provincial Government building at the heart of Novi Sad. The race begins with a neutralized start, leading out of the city via the Rainbow Bridge. Upon crossing to the Srem side, participants trace the scenic path along the Danube River until reaching Beočin.

At the 19 km mark, the first ascent commences—a 9 km climb featuring an average gradient of 4.5%, with a maximum gradient of 8%. Following a thrilling descent through Mala Remeta and Jazak villages, the route proceeds to Vrdnik. In Vrdnik, the course turns towards Irig, where the second climb awaits at the 55 km mark. This 6 km ascent boasts an average gradient of 3.8%, reaching a maximum gradient of 7%.

After the top of the second climb, the Gran Fondo traverses the Fruška Gora ridge for 11 km, a pivotal section that may well determine the race outcome. This stretch offers no respite, with the terrain consistently alternating between uphill and downhill. Subsequently, the route returns to the starting point, descending the opposite way from the top of the initial climb. The final 2 km provide a straight and wide path, ideal for exhilarating sprint finishes.